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vizibile provocate de substanță testată. 3. BIBLIOGRAFIE (1) Solbe J. F. de LG (1987). Environmental Effects of Chemicals (CFM 9350 SLD). Report on a UK Ring Test of a Method for Studying the Effects of Chemicals on the Growth Rate of Fish. WRc Report No PRD 1388-M/2. (2) Meyer, A., Bierman, C. H. and Orți, G. (1993). The phylogenetic position of the zebrafish (Danio rerio), a model system în developmental biology: an invitation to the comparative method. Proc. R.

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
position of the zebrafish (Danio rerio), a model system în developmental biology: an invitation to the comparative method. Proc. R. Șoc. Lond. B. 252, pp. 231-236. (3) Ashley S., Mallett M. J. and Grandy N. J. (1990). EEC Ring Test of a Method for Determining the Effects of Chemicals on the Growth Rate of Fish. Final Report to the Commission of the European Communities. WRc Report No EEC 2600-M. (4) Crossland N. O. (1985). A method to evaluate effects of

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
invitation to the comparative method. Proc. R. Șoc. Lond. B. 252, pp. 231-236. (3) Ashley S., Mallett M. J. and Grandy N. J. (1990). EEC Ring Test of a Method for Determining the Effects of Chemicals on the Growth Rate of Fish. Final Report to the Commission of the European Communities. WRc Report No EEC 2600-M. (4) Crossland N. O. (1985). A method to evaluate effects of toxic chemicals on fish growth. Chemosphere, 14, pp. 1855-1870. (5) Nagel R., Bresh

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
of a Method for Determining the Effects of Chemicals on the Growth Rate of Fish. Final Report to the Commission of the European Communities. WRc Report No EEC 2600-M. (4) Crossland N. O. (1985). A method to evaluate effects of toxic chemicals on fish growth. Chemosphere, 14, pp. 1855-1870. (5) Nagel R., Bresh H., Caspers N., Hansen P. D., Market M., Munk R., Scholz N. and Höfte B. B. (1991). Effect of 3,4-dichloroaniline on the early life stages of

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
N. O. (1985). A method to evaluate effects of toxic chemicals on fish growth. Chemosphere, 14, pp. 1855-1870. (5) Nagel R., Bresh H., Caspers N., Hansen P. D., Market M., Munk R., Scholz N. and Höfte B. B. (1991). Effect of 3,4-dichloroaniline on the early life stages of the Zebrafish (Brachydanio rerio): results of a comparative laboratory study. Ecotox. Environ. Safety, 21, pp. 157-164. (6) Yamamoto, Tokio. (1975). Series of stock cultures în biological field. Medaka (killifish) biology and strains

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
Chemosphere, 14, pp. 1855-1870. (5) Nagel R., Bresh H., Caspers N., Hansen P. D., Market M., Munk R., Scholz N. and Höfte B. B. (1991). Effect of 3,4-dichloroaniline on the early life stages of the Zebrafish (Brachydanio rerio): results of a comparative laboratory study. Ecotox. Environ. Safety, 21, pp. 157-164. (6) Yamamoto, Tokio. (1975). Series of stock cultures în biological field. Medaka (killifish) biology and strains. Keigaku Publish. Tokio, Japan. (7) Holcombe, G. W., Benoit D. A., Hammermeister, D. E., Leonard, E. N

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
157-164. (6) Yamamoto, Tokio. (1975). Series of stock cultures în biological field. Medaka (killifish) biology and strains. Keigaku Publish. Tokio, Japan. (7) Holcombe, G. W., Benoit D. A., Hammermeister, D. E., Leonard, E. N. and Johnson, R. D. (1995). Acute and long-term effects of nine chemicals on the Japanese medaka (Oryzias latipes). Arch. Environ. Conta. Toxicol. 28, pp. 287-297. (8) Benoit, D. A., Holcombe, G. W. and Spehar, R. L. (1991). Guidelines for conducting early life toxicity tests with Japanese medaka (Oryzias latipes). Ecological Research

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
1991). Guidelines for conducting early life toxicity tests with Japanese medaka (Oryzias latipes). Ecological Research Seriese PA-600/3-91-063. USE nvironmental Protection Agency, Duluth, Minnesota. (9) Stephan C. E. and Rogers J. W. (1985). Advantages of using regression analysis to calculate results of chronic toxicity tests. Aquatic Toxicology and Hazard Assessment: Eighth Symposium, ASTM STP 891, R. C. Bahner and D. J. Hansen, eds., American Society for Testing and Materials, Philadelphia, pp. 328-338. (10) Environment Canada (1992). Biological test method: toxicity tests using

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10) Environment Canada (1992). Biological test method: toxicity tests using early life stages of salmonid fish (rainbow trout, coho salmon, or atlantic salmon). Conservation and Protection, Ontario, Report EPS 1/RM/28, 81 pp. (11) Cox D. R. (1958). Planning of experiments. Wiley Edt. (12) Pack S. (1991). Statistical issues concerning the design of tests for determining the effects of chemicals on the growth rate of fish. Room Document 4, OECD Ad Hoc Meeting of Experts on Aquatic Toxicology, WRc Medmenham

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salmon, or atlantic salmon). Conservation and Protection, Ontario, Report EPS 1/RM/28, 81 pp. (11) Cox D. R. (1958). Planning of experiments. Wiley Edt. (12) Pack S. (1991). Statistical issues concerning the design of tests for determining the effects of chemicals on the growth rate of fish. Room Document 4, OECD Ad Hoc Meeting of Experts on Aquatic Toxicology, WRc Medmenham, UK, 10-12 December 1991. (13) Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with a

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Protection, Ontario, Report EPS 1/RM/28, 81 pp. (11) Cox D. R. (1958). Planning of experiments. Wiley Edt. (12) Pack S. (1991). Statistical issues concerning the design of tests for determining the effects of chemicals on the growth rate of fish. Room Document 4, OECD Ad Hoc Meeting of Experts on Aquatic Toxicology, WRc Medmenham, UK, 10-12 December 1991. (13) Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with a Control, J. Amer. Statist. Assoc., 50

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with a zero dose control. Biometrics 27, pp. 103-117. (16) Johnston, W. L., Atkinson, J. L., Glanville N. Ț. (1994). A technique using sequential feedings of different coloured food to determine food intake by individual rainbow trout, Oncorhynchus mykiss: effect of feeding level. Aquaculture 120, pp. 123-133. (17) Quinton, J. C. and Blake, R. W. (1990). The effect of feed cycling and ration level on the compensatory growth response în rainbow trout, Oncorhynchus mykiss. Journal of Fish Biology, 37, pp. 33-41

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Ț. (1994). A technique using sequential feedings of different coloured food to determine food intake by individual rainbow trout, Oncorhynchus mykiss: effect of feeding level. Aquaculture 120, pp. 123-133. (17) Quinton, J. C. and Blake, R. W. (1990). The effect of feed cycling and ration level on the compensatory growth response în rainbow trout, Oncorhynchus mykiss. Journal of Fish Biology, 37, pp. 33-41. (22) Post, G. (1987). Nutrition and Nutrițional Diseases of Fish. Chapter IX în Testbook of Fish Health. T.F.H.

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American Petroleum Institute Publication, No 4468). Electric Power Research Institute, Palo Alto, CA. (22) Norbert-King Ț. J. (1988). An interpolation estimate for chronic toxicity: the Icp approach. USE nvironmental Protection Agency. Environmental Research Lab., Duluth, Minnesota. Tech. Rep. No 05-88 of Național Effluent Toxicity Assesment Center. Sept. 1988. 12 pp. (22) Williams D. A. (1972). The comparison of several dose levels with a zero dose control. Biometrics 28, pp. 510-531. APENDICE 1 SPECIILE DE PEȘTI RECOMANDATE PENTRU TESTARE ȘI CONDIȚIILE DE LUCRU

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J. (1988). An interpolation estimate for chronic toxicity: the Icp approach. USE nvironmental Protection Agency. Environmental Research Lab., Duluth, Minnesota. Tech. Rep. No 05-88 of Național Effluent Toxicity Assesment Center. Sept. 1988. 12 pp. (22) Williams D. A. (1972). The comparison of several dose levels with a zero dose control. Biometrics 28, pp. 510-531. APENDICE 1 SPECIILE DE PEȘTI RECOMANDATE PENTRU TESTARE ȘI CONDIȚIILE DE LUCRU ADECVATE Specia Intervalul de temperatură recomandat (0C) Perioadă de expunere la lumină (ore) Intervalul recomandat pentru

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A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, pp. 328-330. (7) Dave G., Damgaard B., Grande M., Martelin J. E., Rosander B. and Viktor Ț. (1987). Ring Test of an Embryo-larval Toxicity Test with Zebrafish (Brachydanio rerio) Using Chromium and Zinc aș Toxicants. Environmental Toxicology and Chemistry, 6, pp. 61-71. (8) Birge J. W., Black J. A. and Westerman A. G. (1985). Short-term Fish and Amphibian Embryo-larval Tests for Determining

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
with Control. J. Amer. Statist. Assoc., 50, pp. 1096-1121. (13) Dunnett C. W. (1964). New Tables for Multiple Comparisons with a Control. Biometrics, 20, pp. 482-491. (14) Mc Clave J. Ț., Sullivan J. H. and Pearson J.G. (1980). Statistical Analysis of Fish Chronic Toxicity Test Dată. Proceedings of 4th Aquatic Toxicology Symposium, ASTM, Philadelphia. (15) Van Leeuwen C. J., Adema D. M. M. and Hermes J. (1990). Quantitative Structure-Activity Relationships for Fish Early Life Stage Toxicity. Aquatic Toxicology, 16, pp. 321-334. (16

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Salmon or Atlantic Salmon). Biological Test Method Series. Report EPS 1/RM/28, December 1992, 81 pp. (17) Dave G. and Xiu R. (1991). Toxicity of Mercury, Nickel, Lead and Cobalt to Embryos and Larvae of Zebrafish, Brachydanio rerio. Arch. of Environmental Contamination and Toxicology, 21, pp. 126-134. (18) Meyer A., Bierman C. H. and Orți G. (1993). The phylogenetic position of the Zebrafish (Danio rerio), a model system în developmental biology - an invitation to the comperative methods. Proc. Royal Society

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rerio), a model system în developmental biology - an invitation to the comperative methods. Proc. Royal Society of London, Series B, 252: pp. 231-236. (19) Ghillebaert F., Chaillou C., Deschamps F. and Roubaud P. (1995). Toxic Effects, at Three pH Levels, of Two Reference Molecules on Common Carp Embryo. Ecotoxicology and Environmental Safety 32, pp. 19-28. (20) UȘ EPA, (1991). Guidelines for Culturing the Japanese Medaka, Oryzias latipes. EPA report EPA/600/3-91/064, Dec. 1991, EPA, Duluth. (21) UȘ EPA, (1991

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pp. 1189-1203. (23) Calow P. (1993). Handbook of Ecotoxicology, Blackwells, Oxford. Vol. 1, Chapter 10: Methods for spawning, culturing and conducting toxicity tests with Early Life stages of Estuarine and Marine fish. (24) Balon E. K. (1985). Early life history of fishes: New developmental, ecological and evolutionary perspectives, Junk Publ., Dordrecht, 280 pp. (25) Blaxter J. H. S. (1988). Pattern and variety în development, în: W. S. Hoar and D. J. Randall eds., Fish Physiology, Vol. XIA, Academic press, pp. 1-58

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Comparisons with a Control. Biometrics, 20, pp. 482-491. (8) Williams D. A. (1971). A Test for Differences Between Treatment Means when Several Dose Levels are Compared with a Zero Dose Control. Biometrics, 27, pp. 103-117. (9) Williams D. A. (1972). The Comparison of Several Dose Levels with a Zero Dose Control. Biometrics 28, pp. 519-531. (10) Sokal R. R. and Rohlf F. J. (1981). Biometry, the Principles and Practice of Statistics în Biological Research, W. H. Freeman and Co., Sân Francisco. APENDICE 2

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
Zero Dose Control. Biometrics, 27, pp. 103-117. (9) Williams D. A. (1972). The Comparison of Several Dose Levels with a Zero Dose Control. Biometrics 28, pp. 519-531. (10) Sokal R. R. and Rohlf F. J. (1981). Biometry, the Principles and Practice of Statistics în Biological Research, W. H. Freeman and Co., Sân Francisco. APENDICE 2 CONDIȚIILE, DURATA TESTULUI ȘI CRITERIILE DE SUPRAVIEȚUIRE PENTRU SPECIILE RECOMANDATE Specia Temperatura (oC) Salinitatea (0/00) Perioadă de expunere la lumină (ore) Durată etapelor Durată normală a

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dozei cu substanță testată), rata consumului de hrană la grupele tratate și netratate, - orice abatere de la modul de lucru descris și orice alte date relevante. 3. BIBLIOGRAFIE (1) EPPO/Council of Europe (1993). Decision-Making Scheme for the Environmental Risk Assessment of Plant Protection Products ó Honeybees. EPPO Bulletin, Vol. 23, N.1, pp. 151-165. March 1993. (2) Gough, H. J., McIndoe, E.C., Lewis, G.B. (1994). The use of dimethoate aș a reference compound în laboratory acute toxicity tests on honeybees (Apis

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E.C., Lewis, G.B. (1994). The use of dimethoate aș a reference compound în laboratory acute toxicity tests on honeybees (Apis mellifera L.) 1981-1992. Journal of Apicultural Research, 22, pp. 119-125. (3) Litchfield, J.T. and Wilcoxon, F. (1949). A simplified method of evaluating dose-effect experiments. Jour. Pharmacol. and Exper. Ther., 96, pp. 99-113. (4) Finney, D. J. (1971). Probit Analysis. 3rd ed., Cambridge, London and New York. (5) Abbott, W. S. (1925). A method for computing the effectiveness of an insecticide. Jour. Econ.

jrc5127as2001 by Guvernul României (2001) [Corola-website/Law/90295_a_91082]
1949). A simplified method of evaluating dose-effect experiments. Jour. Pharmacol. and Exper. Ther., 96, pp. 99-113. (4) Finney, D. J. (1971). Probit Analysis. 3rd ed., Cambridge, London and New York. (5) Abbott, W. S. (1925). A method for computing the effectiveness of an insecticide. Jour. Econ. Entomol., 18, pp. 265-267. C.17. ALBINELE - TESTUL DE TOXICITATE ACUTĂ PRIN CONTACT 1. METODĂ Metodă de determinare a toxicității acute reproduce Linia directoare 214 a OCDE (1998). 1.1. INTRODUCERE Prezentul test de toxicitate este

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